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Antibodies bound to the blocking peptide now not bind on the epitope around the concentrate on protein. By comparing the staining from the blocked antibody as opposed to the antibody on your own, you can see which staining is distinct.
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This information outlines even more probable optimization of cellular and immunoassay parameters to be certain the absolute best success are...
This guidebook outlines even further feasible optimization of cellular and immunoassay parameters to be certain the best possible final results are...
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The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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The measurement of protein phosphorylation is a great tool for measuring the modulation of receptor activation by both equally antibodies...
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Blocking peptides are peptides that bind exclusively to your focus on antibody and block antibody binding. These peptide normally includes the epitope acknowledged by the antibody.
Target Information and facts Cyclin E belongs to your really conserved cyclin family, whose users are characterised by a remarkable periodicity in protein abundance throughout the cell cycle. Cyclins functionality as regulators of CDK kinases. Unique cyclins exhibit distinct expression and degradation patterns which add to your temporal coordination of each mitotic party. Cyclin E varieties a posh with and functions as being a regulatory subunit of CDK2, whose action is necessary for mobile cycle G1/S changeover. This protein accumulates on the G1-S phase boundary which is degraded as cells progress by means of S phase.
Overexpression of the gene has long been observed in lots of tumors, which ends up in chromosome instability, and so may well contribute to tumorigenesis. This protein was uncovered to associate with, and become associated with, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in cell-cycle regulated histone gene expression and plays a important position in promoting mobile-cycle progression in the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinctive isoforms, have already been explained. Two extra splice variants ended up reported but comprehensive nucleotide sequence facts isn't nevertheless available.
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